Effect of TSG-6 on Corneal Inflammation After Alkali Injury

Fulcher, Samuel

Effect of TSG-6 on Corneal Inflammation After Alkali Injury

Monday, April 28, 2014: 9:01 AM

Room 154 (Boston Convention and Exhibition Center)

Samuel F.A. Fulcher, MD, Central Texas Veterans Health Care System, Temple, TX, USA

Joo Youn Oh, MD, PhD, Seoul National University Hospital,, Seoul, South Korea

Roxanne L. Reger, MS, Texas A&M HSC COM, Temple, TX, USA

Hosoon Choi, Texas A & M, Temple, TX, USA

Darwin J. Prockop, MD, PhD, Texas A&M Health Science Center COM, Temple, TX, USA

Ji Min Yu Temple, TX, USA

Narrative Responses:

Purpose
Previously the inflammation modulatory protein TSG-6 decreased inflammatory damage to the cornea after chemical injury and scraping (Oh et al., EMBO Mol Med. 2012 May;4(5):435-48). Here we tested TSG-6 after damage to the cornea caused by alkali, a more common form of corneal injury.

Methods
Corneas of mice were exposed for 1 min to filters soaked in 1 M NaOH. TSG-6 was administered topically (0.1 μg/μl in PBS) for 5 min or IV (40 μg in 200 μl PBS).

Results
Neutrophil infiltration as assayed by myeloperoxidase activity was reduced by IV TSG-6 application (p=0.044), but not by topical application of TSG-6.

Conclusion
TSG-6 is probably effective where other agents have failed because it has a unique mode
of action in modulating inflammation. Further investigation is warranted to determine if TSG-6 could be effective in the treatment of chemical injury of the cornea in humans.