Optimizing Fluence Settings and Riboflavin Composition for CXL in Antimicrobial Efficiency Against Pseudomonas Aeruginosa

Saturday, April 26, 2014: 1:36 PM
Room 151B (Boston Convention and Exhibition Center)
Olivier Richoz, MD, Geneva University Hospitals, Geneva, Switzerland
Florence Hoogewoud, MD, Geneva Univversity Hospital, Geneva, Switzerland
Farhad Hafezi, MD, PhD, Geneva University Hospitals, Geneva, Switzerland

Narrative Responses:

Purpose
When treating keratitis, determination of the correct pathogen is often clinically challenging. The benefits of using CXL to treat corneal infections is that the treatment is not pathogen-specific. To optimize the treatment parameters, we analyzed the effect of high fluence CXL on the killing rate in an in vitro model.

Methods
The killing rate of a known concentration of bacterias (Pseudomonas aeruginosa) was analyzed for the following conditions: 1) preservative-free riboflavin, with UV-A irradiation @ 18 mW/cm2 for 5 minutes  2) preservative-free riboflavin, with UV-A irradiation @ 36 mW/cm2 for 2.5 minutes  3) riboflavin with preservatives, with UV-A irradiation @ 18 mW/cm for 5 minutes  4) riboflavin with preservatives, with UV-A irradiation @ 36 mW/cm2 for 2.5 minutes  5) riboflavin only, no UVA  6) riboflavin with preservatives, no UVA. We used 0.1% riboflavin in all experiments.

Results
The groups with preservative-free riboflavin showed a killing rate of 92 % with 18 mW/cm2 and 22 % with 36 mW/cm2. The groups with riboflavine and preservatives showed a killing rate of 2 logs (98 %) with both fluences.

Conclusion
The P. aeruginosa killing rate is fluence-dependent when using conventional riboflavin and fluence-independent when preservatives are added to the riboflavin solution. These findings will allow the generation of optimized riboflavin solutions for the treatment of bacterial keratitis.