Study of Retinal Protective Effect Against Phototoxicity of Colored Acrylic IOLs

Saturday, April 18, 2015: 3:16 PM
Room 1A (San Diego Convention Center)
Kenji Kawai, MD, PhD

Selecting an Colored acrylic intraocular lens (CA-IOL) has become the mainstream for cataract surgery with the hope of their postoperative retinal protective effect.  However, each type of CA-IOL has different spectral transmission characteristics.  In the current study, we comparatively investigated whether the difference in spectral transmission would influence the retinal protective effect.

CA-IOL models including SN60WF (Alcon), NY-60 (HOYA), W-60 (Santen), AN6K (Kowa), NS-60YG (NIDEK), and ZCB00V (AMO) were tested.  Four 20.0 D IOLs of each model were used.  As controls, a group of artificial sunlight irradiation without CA-IOLs and a group of non-irradiation without CA-IOLs were included. Human retinal pigment epithelium cells (APRE-19) were disseminated in a 96-well black plate and cultured for about 24 hours.  Each CA-IOL was placed over the wells, and artificial sunlight (SOL 500, Fuji Film Graphic Systems) was irradiated at the intensity of 14.70 mW/cm2 for 90 minutes.  After irradiation, the cells were cultured for about 24 hours, and the number of viable cells was counted with MTS assay to calculate the viability ratio.

The viability ratio of APRE-19 was 62.4 % in the control group of light irradiation, which was significantly low.  On the other hand, the viability ratio in each IOL model group except ZCB00V was 77.09 - 87.89%, which showed significantly high enough to be effective for retinal protection against phototoxicity.

In comparison with the control, each CA-IOL model presented inhibitory effect against phototoxicity.  However, there was a difference in inhibitory effect depending on the spectral transmission of CA-IOL.  While all the CA-IOLs blocking blue light presented significantly better inhibitory effect, a significant difference was not found between a CA-IOL which transmits a part of blue light wavelength and the control.