Effect of Mitomycin-C on Haze Formation in Rabbit Cornea After PRK: Immunohistochemical Study
Narrative Responses:
Purpose
To investigate the effect of mitomycin-C on haze formation after photorefractive keratectomy (PRK).
Methods
Twenty rabbits underwent bilateral eximer laser PRK to correct -6.00 D of myopia, the right eyes of the 20 rabbits were treated with 0.02 % mitomycin c during surgery. A sponge soaked in mitomycin-C solution was left on the laser-treated rabbit cornea for 5 minutes. (PRK+MMC group) and the left eyes did not recive 0.02% mitomycin-C (PRK alone group) clinical and histopathological examination were performed. The tissues were evaluated immunohistochemically with antibody to α – smooth muscle actine (SMA). Cellular changes in the anterior stroma and epithelial basement membrane were evaluated by light microscopey. Keratocytes were counted in both groups.
Results
Ten rabbits had PRK + MMC (0.02% MMC was placed for 30 seconds). 10 rabbits had PRK without MMC use. The number of keratocytes, as a marker of corneal tissue response to PRK, in the group receiving MMC after PRK was lower than that in the PRK without MMC group, this difference was statistically significant.
Conclusion
Use of MMC with PRK markedly reduce keratocyte proliferation and reduce the incidence and severity of corneal haze.